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FARAFARA Cure FA

Transposon Tn7 Preferentially Inserts into GAA-TTC Triplet Repeats under Conditions Conducive to Y-R-Y Triplex Formation

Expansion of an unstable GAA•TTC repeat in the first intron of the FXN gene causes Friedreich ataxia by reducing frataxin expression. Structure formation by the repeat has been implicated in both frataxin repression and GAA•TTC instability. The GAA•TTC sequence is capable of adopting multiple non-B DNA structures including Y•R•Y and R•R•Y triplexes. Lower pH promotes the formation of Y•R•Y triplexes by GAA•TTC. Here we used the bacterial transposon Tn7 as an in vitro tool to probe whether GAA•TTC repeats can attract a well-characterized recombinase.

Transposon Tn7 Preferentially Inserts into GAA-TTC Triplet Repeats under Conditions Conducive to Y-R-Y Triplex Formation


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Jen Farmer

Jen Farmer

Executive Director

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