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Electrophysiology of Respiratory Chain Complexes and ADP/ATP Exchanger in Native Mitochondrial Membranes

Transport of protons and solutes across mitochondrial membranes is essential for many physiological processes. However, neither the proton-pumping respiratory chain complexes nor the mitochondrial secondary active solute transport proteins have been characterized electrophysiologically in their native environment. In this study, solid-supported membrane (SSM) technology was applied for electrical measurements of respiratory chain complexes CI, CII, CIII, and CIV, the F(O)F(1)-ATPase/synthase (CV), and the adenine nucleotide translocase (ANT) in inner membranes of pig heart mitochondria.

Electrophysiology of Respiratory Chain Complexes and ADP/ATP Exchanger in Native Mitochondrial Membranes

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